摘要:

Daily injections of peanut oil for 17 or 18 days cause decrease in rate of growth of the rat, and testicular atrophy. Hence results in experiments in which peanut oil (and probably similar oils) are used as solvent vehicles for administration of material by injection may be misinterpreted. Oral administration of oestradiol (3 mgm. daily) to young mature rats for three weeks or more causes decrease in growth rate, relative decrease in size of kidneys, heart, spleen, muscle, and ovaries, little effect on the liver, marked decrease in size of testes, even to actual atrophy, and frequent enlargement of the adrenals in males, with occasional enlargement in females. The adrenals are discoloured to a maroon shade, whether enlarged or not, and evidence is advanced that they are undergoing a pathological change; any enlargement is not in the nature of hypertrophy. Oral administration of stilboestrol gives similar results. The general effects of oestrogens are more marked in male than in female animals. Loss of appetite and diminished food intake are among the general effects, but the mild chronic starvation so produced can only contribute in very minor degree to the other oestrogenic effects.In mild chronic starvation from food restriction the liver is invariably affected, while the adrenals are never enlarged nor discoloured.The effects of combined oestrogenic and thyroid administration seem to be neither additive nor truly antagonistic.

ISSN:1923-4287    

DOI:10.1139/cjr46e-012

出版商:NRC Research Press    

年代:1946

数据来源: NRC

摘要:

The present communication deals with the effect of reducing agents (cysteine hydrochloride, sodium thioglycollate, and sodium formaldehyde sulphoxylate) on the viability of equine encephalomyelitis virus (Eastern type). Cysteine hydrochloride was found to be a valuable reagent inin vitrostudies of equine encephalomyelitis virus, because it greatly retards loss of infectivity of the virus under experimental conditions. It was observed that a virus suspension containing cysteine hydrochloride (1:1000) remained infective after exposure to 37 °C. for 14 days, although the guinea-pig titre was reduced from 1:108to 1:102. The same reduction in the titre of virus suspended in buffered 0.85% sodium chloride solution occurred after exposure to 37 °C. for 120 hr. only, and in unbuffered 0.85% sodium chloride after 24 hr. exposure. Further, equine encephalomyelitis virus, in the presence of cysteine hydrochloride, retains its infectivity without demonstrable loss, over a pH range between 4.8 and 8.2 for 48 hr. at 37 °C. The titre of equine encephalomyelitis virus is maintained at 37 °C. for 48 hr. in a rather wide range of Eh, created with the aid of cysteine hydrochloride, at least in the range between − 0.151 and + 0.02 volts. On the other hand, addition of sodium formaldehyde sulphoxylate or sodium thioglycollate to a suspension of equine encephalomyelitis virus does not retard loss of infectivity of the virus. These reagents, therefore, are not suitable for the conservation of infectivity of the virusin vitro.

ISSN:1923-4287    

DOI:10.1139/cjr46e-013

出版商:NRC Research Press    

年代:1946

数据来源: NRC