摘要:

With acridinorange it is possible to stain selectively trypanosomes in blood intra-vitam. In subsequent examination by means of a fluorescence microscope only leucocytes and trypanosomes are visible (as shining bright green bodies), while the erythrocytes are not visible. Living and dead trypanosomes, too, may be distinguished in this way.With the fluorescent dye auramine, trypanosomes in blood may be selectively stained (showing up in golden fluorescence) so that diagnosis for trypanosomes is very greatly facilitated. Even in difficult cases a decision on the presence of trypanosomes in blood is possible.

ISSN:1923-4287    

DOI:10.1139/cjr48e-010

出版商:NRC Research Press    

年代:1948

数据来源: NRC

摘要:

Sulphones in trace quantities combined with diet rich in fresh vegetables produce an increased sensitivity to insulin, both in rate of fall of blood sugar levels and maintenance of hypoglycaemia. The sensitivity is not contingent on the presence of sulphone compounds and may persist for several days after these compounds have been detoxicated or eliminated.

ISSN:1923-4287    

DOI:10.1139/cjr48e-011

出版商:NRC Research Press    

年代:1948

数据来源: NRC

摘要:

Observations have been made on methods for the determination of acetate and acyl phosphate in tissue suspensions. Previous work on the formation of acetate by respiring brain suspensions, especially in the presence of added pyruvate, has been confirmed. No evidence could be obtained that the substance formed is actually acetyl phosphate, which breaks down to yield acetic acid in the course of the estimation or enzymatically during the incubation of the tissue. The amount of acetate formed in brain suspensions is increased by the presence of malonate. It seems also to be increased by the addition of malate or α-ketoglutarate and by thiamine. Spontaneous breakdown of pyruvate could account for part of the acetate formed from pyruvate added to tissue. Fluoroacetic acid interferes with the determination of acetic acid. There was no evidence that it increases the formation of acetate by rat brain tissue suspensions. It does not affect the oxygen uptake of these suspensions with glucose or pyruvate as substrates.

ISSN:1923-4287    

DOI:10.1139/cjr48e-012

出版商:NRC Research Press    

年代:1948

数据来源: NRC

摘要:

Though the drinking of undiluted sea water by the castaway has been shown to be dangerous, there have been few studies on the use of sea water diluted with fresh water. The experiment was designed to permit a statistical evaluation of relative gain or relative loss in body weights to human subjects, from drinking a daily supplement of sea water. The addition of 10 oz. of sea water to the daily ration of 16 oz. of fresh water resulted in a mean (corrected) weight saving of 2.24 lb. over a period of six days. This result was significant. That this relative gain in weight was beneficial is supported by the subjects' unanimous opinion to this effect, by the specific gravities of blood and urine, and by the figures for blood and urine electrolytes in control and experimental subjects.

ISSN:1923-4287    

DOI:10.1139/cjr48e-013

出版商:NRC Research Press    

年代:1948

数据来源: NRC