摘要:

A method of typingH.influenzaeby the precipitin reaction is described. The procedure consists of dissolving the bacterial culture in 90% phenol to destroy the specificity of the somatic proteins followed by alcoholic precipitation of the denatured proteins and type specific polysaccharide. The carbohydrate is extracted from the precipitate with saline and portions of the saline extract added to samples of the six type specific antisera. A positive test is indicated only by a marked turbidity, which develops within five minutes after mixing and denotes the extraction of at least 0.01 mgm. of polysaccharide. A strong positive test is, of course, regularly obtained in the case of cultures shown to be encapsulated by the usual capsular swelling technique. However, the method was devised as an attempt to ascertain whether non-typable (by the capsular swelling technique) derivatives of strains of known type or non-typable, but suspected respiratory pathogens, isolated from clinical material, contained detectable quantities of any of the known type specific polysaccharides.

ISSN:1923-4287    

DOI:10.1139/cjr48e-006

出版商:NRC Research Press    

年代:1948

数据来源: NRC

摘要:

It has been shown that salicylic acid and gentisic acid, a substance formed in the organism from salicylic acid, have no influence on the activity of hyaluronidasein vitro. However carboxy-p-benzoquinone, the quinone corresponding to gentisic acid, was found to inhibit the enzymein vitro.

ISSN:1923-4287    

DOI:10.1139/cjr48e-007

出版商:NRC Research Press    

年代:1948

数据来源: NRC

摘要:

Glucose excretion and dextrose: nitrogen ratios were measured following phlorizination in young fasting rats depleted of pyridoxine and in their pair-fed controls. The same determinations were also made in older and non-fasting animals following a period of 11 to 16 weeks on a sugar-free, pyridoxine-free ration. Pair-fed rats were again used as controls. No significant differences were found between groups. It is concluded that under the experimental conditions described, pyridoxine insufficiency does not exert a specific effect on gluconeogenesis.

ISSN:1923-4287    

DOI:10.1139/cjr48e-008

出版商:NRC Research Press    

年代:1948

数据来源: NRC

摘要:

Kidney lesions resembling those of chronic nephritis and nephrosclerosis developed in rats treated with L.A.P. (lyophilized anterior pituitary) and fed 'Purina', but did not develop in similar rats similarly treated and fed Purina mixed with cornstarch in a 1: 1 ratio. When synthetic diets, differing only in their relative carbohydrate and protein content, were fed, only those L.A.P.-treated rats that consumed a regimen containing 30% casein and 54% cornstarch developed nephrosclerosis. No such lesions were seen in treated rats kept on a 15% casein and 69% cornstarch diet. This difference in response is due to the protein and not to the cornstarch content of these diets; the entire amount of cornstarch was substituted by wheat starch or 15% of it was replaced by an equicaloric amount of fat, without influencing the development of kidney lesions.Various protein preparations differed in their ability to cause kidney damage. Casein, egg albumen, and wheat gluten were more damaging than lactalbumin, gelatin, or zein.The severity of nephrosclerosis caused by L.A.P. roughly paralleled the kidney hypertrophy, adrenal enlargement, and increased appetite for water, but not necessarily the food intake or the growth rate. The appearance of these renal lesions was not associated with any gross change in urine pH, but was preceded by a marked albuminuria. By the fifth day, large amounts of albumin were present in the urine of the majority of treated rats consuming the 30% casein diet. Albumin rarely appeared in the urine of treated rats consuming the 15% casein diet.These findings were discussed in relation to the literature concerning the dietary production of chronic nephritis. The procedure described in this paper greatly accelerates the development of kidney lesions, apparently identical with those shown previously to ensue on the feeding of high protein diets.

ISSN:1923-4287    

DOI:10.1139/cjr48e-009

出版商:NRC Research Press    

年代:1948

数据来源: NRC