摘要:

Three methods of staining myelin sheaths by the Weigert-Pal method in paraffin sections are compared. Hyomandibular nerves and spinal cord of the ratfish (Hydrolagus colliei) and tibial and peroneal nerves of the white rat were used. Fixation and mordanting comprised: 10% neutral formol, 48 hr.; 2.5% potassium bichromate at 37 °C., 12 days; gliabeze, 2 days. The tissue was washed for 24 hr., and stained for 24 hr. either in bulk, or while in paraffin sections, or on the slide after coating with celloidin. Kulschitzky's haematoxylin, freshly prepared and artificially ripened, was used for staining. Differentiation was carried out by Pal's method. Bulk staining gave most satisfactory results, with the other methods as second and third alternatives, respectively. Treating the sections with safranin and dehydrating in dioxan was found to give a satisfactory counterstain.

ISSN:1923-4287    

DOI:10.1139/cjr49d-001

出版商:NRC Research Press    

年代:1949

数据来源: NRC

摘要:

Several methods were tried of decalcifying material to be stained by the Weigert-Pal method for myelin sheaths. Pieces of the tail of a teleost,Porichthys notatus, and peroneal and tibial nerves of the white rat were used. The following procedure was found to give good staining of myelin sheaths after treatment with acid. Fix tissue in 10% neutral formol. Transfer to 5% trichloroacetic acid for five days. Wash one hour, and mordant in potassium bichromate followed by gliabeze, Prepare paraffin sections, stain with Kulschitzky's hematoxylin, and differentiate by Pal's method.

ISSN:1923-4287    

DOI:10.1139/cjr49d-002

出版商:NRC Research Press    

年代:1949

数据来源: NRC

摘要:

The varying susceptibility and availability of game fish populations to angling methods is seldom approached by modern scientific procedure, despite the fact that angling returns are generally accepted as reliable indices of population densities. Lake Simcoe—Ontario's fourth largest lake—afforded an excellent opportunity to study a situation of long standing wherein a great abundance of smallmouth bass had long yielded generally poor angling returns.Results from a scientific investigation, conducted mostly during the summer of 1940, were as follows:(1) A somewhat radical angling method was developed which yields greatly increased and highly satisfactory returns from the bass population.(2) The discovery that shifts in habitat—both diurnal and otherwise—by particular age groups of the smallmouth bass population had a significant effect upon angling returns.(3) The discovery that the smallmouth bass population of the lake was heterogeneous in character, i.e. that bass from one section of the lake had a significantly more rapid rate of growth than bass from another section, and that this influenced the quality of catch.(4) The conclusion that the smallmouth bass problem of Lake Simcoe cannot be solved by stocking; but rather by the dissemination of knowledge on improved methods of utilization.

ISSN:1923-4287    

DOI:10.1139/cjr49d-003

出版商:NRC Research Press    

年代:1949

数据来源: NRC