摘要:

A simple method for the quantitative determination of small amounts of diphtheria antitoxin is presented. Toxin of known strength is mixed with various dilutions of serum. These are held at room temperature for two hours and 0.1 cc. of each dilution is injected intracutaneously into the back of a suitably prepared rabbit. The resulting skin reactions are compared with reactions obtained with control mixtures, containing known amounts of antitoxin, injected at the same time. Between 200 and 300 tests may be performed on the back of a single rabbit.

ISSN:1923-4287    

DOI:10.1139/cjr49e-001

出版商:NRC Research Press    

年代:1949

数据来源: NRC

摘要:

Using the temperature of the digits to measure vasodilation, the dilating effect of tetraethylammonium chloride (Etamon) was compared to that of thermal stimulation. It was found in three normal subjects and 11 patients that doses of 300 to 500 mgm. administered intravenously never induced a higher skin temperature than did body heating and the mean maximum temperature was 5° to 6 °C. lower. Because of the hypotensive effect of the drug higher doses were not used. It was concluded that Etamon was not a reliable agent for testing the vasodilatation potential of the peripheral circulation.

ISSN:1923-4287    

DOI:10.1139/cjr49e-002

出版商:NRC Research Press    

年代:1949

数据来源: NRC

摘要:

There is a conflict of evidence concerning the alterations of nondiffusible plasma magnesium in disorders of the thyroid gland. In this investigation it was found in 12 normal individuals, 9 patients with hyperthyroidism, 10 with myxedema, 6 with nontoxic goiter, and 14 patients with miscellaneous diseases, that the nondiffusible plasma magnesium was relatively constant in a wide range of conditions and was not altered even in patients with marked thyroid dysfunction.

ISSN:1923-4287    

DOI:10.1139/cjr49e-003

出版商:NRC Research Press    

年代:1949

数据来源: NRC

摘要:

The urinary excretion of 17-ketosteroids was measured during the first four hours after waking and during the next 20 hr. of the day in 29 ill adults, 18 males and 11 females, and in 31 control adults, 16 males and 15 females. The ill subjects excreted a smaller fraction of their total daily output in the first four hours of the day than did the controls. This finding is ascribed to defective secretion of 17-ketosteroid precursors by the adrenal cortex.

ISSN:1923-4287    

DOI:10.1139/cjr49e-004

出版商:NRC Research Press    

年代:1949

数据来源: NRC

摘要:

A simple and effective method for preservation of acetylcholine in human cerebrospinal fluid has been described. The method depends on the use of acidification and prostigminization of cerebrospinal fluid. No detectable loss of acetylcholine for periods up to seven days or longer has been observed. The method has been satisfactorily used in clinical investigations on more than 200 cerebrospinal fluid samples from over 100 neurological and neurosurgical patients.

ISSN:1923-4287    

DOI:10.1139/cjr49e-005

出版商:NRC Research Press    

年代:1949

数据来源: NRC

摘要:

It has been found that, when weanling rats are placed on a diet containing low protein barley (protein = 8.5%) or on diets containing low protein barley with supplements of casein or individual essential amino acids, with the exception of lysine and methionine, the level of serum alkaline phosphatase is related in an inverse fashion to the rate of growth. The addition to the basal low protein diet of methionine or casein, i.e., of labile methyl groups, results in the lowering of serum alkaline phosphatase activity towards normal levels. This is considered to reflect a return to normal of fat mobilization.

ISSN:1923-4287    

DOI:10.1139/cjr49e-006

出版商:NRC Research Press    

年代:1949

数据来源: NRC

摘要:

The possibilities of producing isinglass solution in sufficiently large quantity to permit adequate clinical trial on a uniform batch have been investigated. A satisfactory process has been developed using fresh frozen hake sounds. Technical details of this preparation are presented in this paper, together with comments on the occurrence and prevention of pyrogens in transfusion fluids. The formation of pyrogens was a continual source of trouble until sterile technique for handling large volumes of material was developed. Technical details are presented, including a method for the removal of pyrogenic substances when, in spite of precautions, these are present. Preparations free from pyrogenic substances can be produced through the use of aseptic technique or by filtration through sterilizing filter pads. In using the latter method it was necessary to limit the quantity of liquid that was filtered through any single pad.

ISSN:1923-4287    

DOI:10.1139/cjr49e-007

出版商:NRC Research Press    

年代:1949

数据来源: NRC

摘要:

A vaccine made from vole bacilli killed by ultraviolet light has been shown to confer a significant degree of protection against experimental tuberculosis in guinea pigs. The degree of protection was comparable to that obtained from BCG. The results of this single experiment suggest that further work on this material should be carried out.

ISSN:1923-4287    

DOI:10.1139/cjr49e-008

出版商:NRC Research Press    

年代:1949

数据来源: NRC

摘要:

The mean values and standard deviations for the concentrations of the hyaluronic acid and chondroitin sulphuric acid fractions isolated from 11 specimens of human skin were 24.5 ± 5.7 and 26.2 ± 4.7 mgm. per 100 gm. of fresh tissue, respectively.The individual differences in the concentrations of these substances were masked by the technical errors. The small variations in moisture and ash content of the skin did not contribute appreciably to the uncertainty of the concentration values. The purity of the products, determined by their amino sugar content, approximated 60%, which value compared favorably with those obtained by other workers.The content of hexosamine and uronic acid identified both fractions as acid mucopolysaccharides. The release of reducing substances, including N-acetyl hexosamine, from the hyaluronic acid fraction by hyaluronidase paralleled the action of the enzyme upon hyaluronate from umbilical cord. The chondroitin sulphuric acid fraction, which was relatively resistant to the action of hyaluronidase, was assumed to be identical with the chondroitin sulphatelike substance that Meyer and Chaffee isolated from pig skin.

ISSN:1923-4287    

DOI:10.1139/cjr49e-009

出版商:NRC Research Press    

年代:1949

数据来源: NRC